variable name typo
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		| @@ -3,12 +3,13 @@ | ||||
| # Purpose:  A Bioinformatics Course: | ||||
| #              R code accompanying the BIN-PHYLO-Tree_analysis unit. | ||||
| # | ||||
| # Version:  1.0.1 | ||||
| # Version:  1.0.2 | ||||
| # | ||||
| # Date:     2017  10  31 | ||||
| # Author:   Boris Steipe (boris.steipe@utoronto.ca) | ||||
| # | ||||
| # Versions: | ||||
| #           1.0.2  Typo in variable name, style changes | ||||
| #           1.0.1  Wrong section heading | ||||
| #           1.0    First 2017 version | ||||
| #           0.1    First code copied from 2016 material. | ||||
| @@ -29,17 +30,17 @@ | ||||
| #TOC> ========================================================================== | ||||
| #TOC> | ||||
| #TOC>   Section  Title                        Line | ||||
| #TOC> ------------------------------------------- | ||||
| #TOC>   1        ___Section___                 38 | ||||
| #TOC>   2        Tree Analysis                 77 | ||||
| #TOC>   2.1      Rooting Trees                136 | ||||
| #TOC>   2.2      Rotating Clades              182 | ||||
| #TOC>   2.3      Computing tree distances     229 | ||||
| #TOC> -------------------------------------------- | ||||
| #TOC>   1        Preparation and Tree Plot      43 | ||||
| #TOC>   2        Tree Analysis                  82 | ||||
| #TOC>   2.1      Rooting Trees                 141 | ||||
| #TOC>   2.2      Rotating Clades               187 | ||||
| #TOC>   2.3      Computing tree distances      234 | ||||
| #TOC> | ||||
| #TOC> ========================================================================== | ||||
|  | ||||
|  | ||||
| # =    1 Preparation and Tree Plot  ============================================ | ||||
| # =    1  Preparation and Tree Plot  =========================================== | ||||
|  | ||||
|  | ||||
| if (!require(Rphylip, quietly=TRUE)) { | ||||
| @@ -71,8 +72,8 @@ for (i in seq_along(fungiTree$tip.label)) { | ||||
| } | ||||
|  | ||||
| # Plot the tree | ||||
| plot(fungiTree, cex=1.0, root.edge=TRUE, no.margin=TRUE) | ||||
| nodelabels(text=orgTree$node.label, cex=0.6, adj=0.2, bg="#D4F2DA") | ||||
| plot(fungiTree, cex = 1.0, root.edge = TRUE, no.margin = TRUE) | ||||
| nodelabels(text = fungiTree$node.label, cex = 0.6, adj = 0.2, bg = "#D4F2DA") | ||||
| # Note that you can use the arrow buttons in the menu above the plot to scroll | ||||
| # back to plots you have created earlier - so you can reference back to the | ||||
| # species tree. | ||||
| @@ -102,15 +103,15 @@ nodelabels(text=orgTree$node.label, cex=0.6, adj=0.2, bg="#D4F2DA") | ||||
| load(file = "APSEStreeRproml.RData") | ||||
|  | ||||
| plot(apsTree) # default type is "phylogram" | ||||
| plot(apsTree, type="unrooted") | ||||
| plot(apsTree, type="fan", no.margin = TRUE) | ||||
| plot(apsTree, type = "unrooted") | ||||
| plot(apsTree, type = "fan", no.margin = TRUE) | ||||
|  | ||||
| # rescale to show all of the labels: | ||||
| # record the current plot parameters by assigning them to a variable ... | ||||
| (tmp <- plot(apsTree, type="fan", no.margin = TRUE, plot=FALSE)) | ||||
| # ... and adjust the plot limits for a new plot: | ||||
| plot(apsTree, | ||||
|      type="fan", | ||||
|      type = "fan", | ||||
|      x.lim = tmp$x.lim * 1.8, | ||||
|      y.lim = tmp$y.lim * 1.8, | ||||
|      cex = 0.8, | ||||
| @@ -150,8 +151,8 @@ is.rooted(apsTree) | ||||
| plot(apsTree) | ||||
|  | ||||
| # add labels for internal nodes and tips | ||||
| nodelabels(cex=0.5, frame="circle") | ||||
| tiplabels(cex=0.5, frame="rect") | ||||
| nodelabels(cex = 0.5, frame = "circle") | ||||
| tiplabels(cex = 0.5, frame = "rect") | ||||
|  | ||||
| # The outgroup of the tree is tip "11" in my sample tree, it may be a different | ||||
| # number in yours. Substitute the correct node number below for "outgroup". | ||||
| @@ -170,8 +171,8 @@ apsTree$edge.length | ||||
| # to show how it connects to the tree without having an | ||||
| # overlap. | ||||
| apsTree$edge.length[1] <- 0.1 | ||||
| plot(apsTree, cex=0.7) | ||||
| nodelabels(text="MRCA", node=12, cex=0.5, adj=0.1, bg="#ff8866") | ||||
| plot(apsTree, cex = 0.7) | ||||
| nodelabels(text = "MRCA", node = 12, cex = 0.5, adj = 0.1, bg = "#ff8866") | ||||
|  | ||||
|  | ||||
| # This procedure does however not assign an actual length to a root edge, and | ||||
| @@ -179,8 +180,8 @@ nodelabels(text="MRCA", node=12, cex=0.5, adj=0.1, bg="#ff8866") | ||||
| # myself that too. We'll just add a length by hand. | ||||
|  | ||||
| apsTree$root.edge <- mean(apsTree$edge.length) * 1.5 | ||||
| plot(apsTree, cex=0.7, root.edge=TRUE) | ||||
| nodelabels(text="MRCA", node=12, cex=0.5, adj=0.8, bg="#ff8866") | ||||
| plot(apsTree, cex = 0.7, root.edge = TRUE) | ||||
| nodelabels(text = "MRCA", node = 12, cex = 0.5, adj = 0.8, bg = "#ff8866") | ||||
|  | ||||
|  | ||||
| # ==   2.2  Rotating Clades  =================================================== | ||||
| @@ -190,13 +191,13 @@ nodelabels(text="MRCA", node=12, cex=0.5, adj=0.8, bg="#ff8866") | ||||
|  | ||||
| # We can either rotate around individual internal nodes ... | ||||
| layout(matrix(1:2, 1, 2)) | ||||
| plot(apsTree, no.margin=TRUE, root.edge=TRUE) | ||||
| nodelabels(node=17, cex=0.7, bg="#ff8866") | ||||
| plot(rotate(apsTree, node=17), no.margin=TRUE, root.edge=TRUE) | ||||
| nodelabels(node=17, cex=0.7, bg="#88ff66") | ||||
| plot(apsTree, no.margin = TRUE, root.edge = TRUE) | ||||
| nodelabels(node = 17, cex = 0.7, bg = "#ff8866") | ||||
| plot(rotate(apsTree, node = 17), no.margin = TRUE, root.edge = TRUE) | ||||
| nodelabels(node = 17, cex = 0.7, bg = "#88ff66") | ||||
| # Note that the species at the bottom of the clade descending from node | ||||
| # 17 is now plotted at the top. | ||||
| layout(matrix(1), widths=1.0, heights=1.0) | ||||
| layout(matrix(1), widths = 1.0, heights = 1.0) | ||||
|  | ||||
| # ... or we can plot the tree so it corresponds as well as possible to a | ||||
| # predefined tip ordering. Here we use the ordering that phyloT has returned | ||||
| @@ -209,13 +210,13 @@ nOrg <- length(apsTree$tip.label) | ||||
|  | ||||
| layout(matrix(1:2, 1, 2)) | ||||
| plot(fungiTree, | ||||
|      no.margin=TRUE, root.edge=TRUE) | ||||
| nodelabels(text=fungiTree$node.label, cex=0.5, adj=0.2, bg="#D4F2DA") | ||||
|      no.margin = TRUE, root.edge = TRUE) | ||||
| nodelabels(text = fungiTree$node.label, cex = 0.5, adj = 0.2, bg = "#D4F2DA") | ||||
|  | ||||
| plot(rotateConstr(apsTree, apsTree$tip.label[nOrg:1]), | ||||
|      no.margin=TRUE, root.edge=TRUE) | ||||
| add.scale.bar(length=0.5) | ||||
| layout(matrix(1), widths=1.0, heights=1.0) | ||||
|      no.margin = TRUE, root.edge = TRUE) | ||||
| add.scale.bar(length = 0.5) | ||||
| layout(matrix(1), widths = 1.0, heights = 1.0) | ||||
|  | ||||
| # Task: Study the two trees and consider their similarities and differences. | ||||
| #         What do you expect? What do you find? Note that this is not a "mixed" | ||||
|   | ||||
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