Renamed tests subdirectory to mirror source
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12
tests/automlst/engine/local/test_abif.py
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12
tests/automlst/engine/local/test_abif.py
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import os
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from automlst.engine.local.abif import read_abif, reference_consensus_assembly
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async def test_load_sanger_sequence_has_data():
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assert os.path.exists("tests/resources/1I1_F_P1815443_047.ab1")
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result_data = await read_abif("tests/resources/1I1_F_P1815443_047.ab1")
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assert result_data is not None
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async def test_consensus_assembly_with_ncbi():
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consensus = reference_consensus_assembly("ON685494.1", [await read_abif("tests/resources/1I1_F_P1815443_047.ab1"), await read_abif("tests/resources/1I1_R_P1815443_094.ab1")])
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# TODO complete implementing this
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7
tests/automlst/engine/local/test_fasta.py
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7
tests/automlst/engine/local/test_fasta.py
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from automlst.engine.local.fasta import read_fasta
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async def test_fasta_reader_not_none():
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named_strings = read_fasta("tests/resources/tohama_I_bpertussis.fasta")
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async for named_string in named_strings:
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assert named_string.name == "BX470248.1"
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from automlst.engine.remote.databases.ncbi.genbank import fetch_ncbi_genbank
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async def test_fetch_ncbi_genbank_with_id_works():
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assert len((await fetch_ncbi_genbank("CP011448.1")).sequence) > 0
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115
tests/automlst/engine/remote/databases/test_bigsdb.py
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115
tests/automlst/engine/remote/databases/test_bigsdb.py
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from Bio import SeqIO
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from automlst.engine.data.mlst import Allele, MLSTProfile
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from automlst.engine.remote.databases.bigsdb import BIGSdbIndex, BigSDBMLSTProfiler
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async def test_institutpasteur_profiling_results_in_exact_matches_when_exact():
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sequence = str(SeqIO.read("tests/resources/tohama_I_bpertussis.fasta", "fasta").seq)
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async with BigSDBMLSTProfiler(database_api="https://bigsdb.pasteur.fr/api", database_name="pubmlst_bordetella_seqdef", schema_id=3) as dummy_profiler:
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exact_matches = dummy_profiler.fetch_mlst_allele_variants(sequence_string=sequence)
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targets_left = {"adk", "fumC", "glyA", "tyrB", "icd", "pepA", "pgm"}
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async for exact_match in exact_matches:
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assert isinstance(exact_match, Allele)
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assert exact_match.allele_variant == '1' # All of Tohama I has allele id I
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targets_left.remove(exact_match.allele_loci)
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assert len(targets_left) == 0
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async def test_institutpasteur_profiling_results_in_correct_mlst_st():
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async def dummy_allele_generator():
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dummy_alleles = [
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Allele("adk", "1"),
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Allele("fumC", "1"),
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Allele("glyA", "1"),
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Allele("tyrB", "1"),
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Allele("icd", "1"),
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Allele("pepA", "1"),
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Allele("pgm", "1"),
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]
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for dummy_allele in dummy_alleles:
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yield dummy_allele
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async with BigSDBMLSTProfiler(database_api="https://bigsdb.pasteur.fr/api", database_name="pubmlst_bordetella_seqdef", schema_id=3) as dummy_profiler:
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mlst_st_data = await dummy_profiler.fetch_mlst_st(dummy_allele_generator())
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assert mlst_st_data is not None
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assert isinstance(mlst_st_data, MLSTProfile)
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assert mlst_st_data.clonal_complex == "ST-2 complex"
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assert mlst_st_data.sequence_type == "1"
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async def test_institutpasteur_sequence_profiling_is_correct():
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sequence = str(SeqIO.read("tests/resources/tohama_I_bpertussis.fasta", "fasta").seq)
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async with BigSDBMLSTProfiler(database_api="https://bigsdb.pasteur.fr/api", database_name="pubmlst_bordetella_seqdef", schema_id=3) as dummy_profiler:
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profile = await dummy_profiler.profile_string(sequence)
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assert profile is not None
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assert isinstance(profile, MLSTProfile)
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assert profile.clonal_complex == "ST-2 complex"
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assert profile.sequence_type == "1"
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async def test_pubmlst_profiling_results_in_exact_matches_when_exact():
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dummy_alleles = {
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Allele("adk", "1"),
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Allele("atpG", "1"),
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Allele("frdB", "1"),
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Allele("fucK", "1"),
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Allele("mdh", "1"),
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Allele("pgi", "1"),
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Allele("recA", "5"),
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}
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sequence = str(SeqIO.read("tests/resources/FDAARGOS_1560.fasta", "fasta").seq)
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async with BigSDBMLSTProfiler(database_api="https://rest.pubmlst.org/", database_name="pubmlst_hinfluenzae_seqdef", schema_id=1) as dummy_profiler:
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exact_matches = dummy_profiler.fetch_mlst_allele_variants(sequence_string=sequence)
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async for exact_match in exact_matches:
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assert isinstance(exact_match, Allele)
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dummy_alleles.remove(exact_match)
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assert len(dummy_alleles) == 0
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async def test_pubmlst_profiling_results_in_correct_st():
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async def generate_dummy_targets():
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dummy_alleles = [
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Allele("adk", "1"),
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Allele("atpG", "1"),
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Allele("frdB", "1"),
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Allele("fucK", "1"),
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Allele("mdh", "1"),
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Allele("pgi", "1"),
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Allele("recA", "5"),
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]
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for dummy_allele in dummy_alleles:
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yield dummy_allele
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async with BigSDBMLSTProfiler(database_api="https://rest.pubmlst.org/", database_name="pubmlst_hinfluenzae_seqdef", schema_id=1) as dummy_profiler:
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mlst_st_data = await dummy_profiler.fetch_mlst_st(generate_dummy_targets())
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assert mlst_st_data is not None
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assert isinstance(mlst_st_data, MLSTProfile)
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assert mlst_st_data.clonal_complex == "ST-3 complex"
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assert mlst_st_data.sequence_type == "3"
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async def test_pubmlst_sequence_profiling_is_correct():
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sequence = str(SeqIO.read("tests/resources/FDAARGOS_1560.fasta", "fasta").seq)
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async with BigSDBMLSTProfiler(database_api="https://rest.pubmlst.org/", database_name="pubmlst_hinfluenzae_seqdef", schema_id=1) as dummy_profiler:
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profile = await dummy_profiler.profile_string(sequence)
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assert profile is not None
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assert isinstance(profile, MLSTProfile)
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assert profile.clonal_complex == "ST-3 complex"
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assert profile.sequence_type == "3"
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async def test_bigsdb_index_all_databases_is_not_empty():
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async with BIGSdbIndex() as bigsdb_index:
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assert len(await bigsdb_index.get_known_seqdef_dbs()) > 0
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async def test_bigsdb_index_references_pubmlst_correctly():
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async with BIGSdbIndex() as bigsdb_index:
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assert (await bigsdb_index.get_bigsdb_api_from_seqdefdb("pubmlst_hinfluenzae_seqdef")) == "https://rest.pubmlst.org"
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async def test_bigsdb_index_references_institutpasteur_correctly():
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async with BIGSdbIndex() as bigsdb_index:
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assert (await bigsdb_index.get_bigsdb_api_from_seqdefdb("pubmlst_bordetella_seqdef")) == "https://bigsdb.pasteur.fr/api"
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async def test_bigsdb_index_instantiates_correct_profiler():
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sequence = str(SeqIO.read("tests/resources/tohama_I_bpertussis.fasta", "fasta").seq)
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async with BIGSdbIndex() as bigsdb_index:
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async with await bigsdb_index.build_profiler_from_seqdefdb("pubmlst_bordetella_seqdef", 3) as profiler:
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profile = await profiler.profile_string(sequence)
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assert profile.clonal_complex == "ST-2 complex"
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assert profile.sequence_type == "1"
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